Localization of Cell-bound Penicillinase in Bacillus licheniformis
نویسندگان
چکیده
منابع مشابه
Immunoelectron microscopic localization of penicillinase in Bacillus licheniformis.
Penicillinase was localized in log-phase cells of Bacillus licheniformis 749/C by labeling with ferritin-anti-penicillinase immunoglobulin G conjugate. Mildly fixed homogenized cells, isolated subcellular fractions, and frozen thin sections were labeled. The label was distributed in discrete patches in the cell envelope. The patches extended from the inside part of the membrane to the outside p...
متن کاملThe cell-bound penicillinase of Bacillus cereus.
Only 30-50 yo of the cell-bound penicillinase of Bacillus cereus NRRL 569 is neutralized by antiserum prepared against the exo-penicillinase. The unneutralizable fraction is not decreased by cell disintegration which liberates a proportion of the cell-bound enzyme into solution. Absence of neutralization cannot therefore be explained by the existence of a mechanical barrier which prevents acces...
متن کاملCharacteristics of penicillinase release by washed cells of Bacillus licheniformis.
Saline-washed cells of Bacillus licheniformis strain 749/C (constitutive for penicillinase) were able to release exopenicillinase in the presence of concentrations of chloramphenicol that prevented protein synthesis completely. The release reaction was strongly pH-dependent, occurring at a faster rate at alkaline pH in anionic or cationic buffers than at neutral pH. A strongly pH-dependent rele...
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The penicillinase repressor (PENI) negatively regulates expression of the penicillinase gene (penP) in Bacillus licheniformis by binding to its operators located within the promoter region of penP.penI codes for a protein with 128 amino acids. Filter-binding analyses suggest that the active form of the repressor is a dimer. Genetic analyses of PENI derivatives showed that the repressor carrying...
متن کاملPhospholipid metabolism during penicillinase production in Bacillus licheniformis.
During membrane-bound penicillinase production, Bacillus licheniformis forms vesicles and tubules that do not appear in the absence of penicillinase production. The major lipids of B. licheniformis were shown to be phospholipids. The proportions, metabolism, and the total phospholipid per cell were shown to be essentially the same in the uninduced control, induced and constitutive penicillinase...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1968
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.96.4.1329-1338.1968